QUALICHEK Vitamin B9 (Biotin) ELISA

QUALICHEK Vitamin B9 (Biotin) ELISA

₹41360

Enzyme Immunoassay for the estimation of Vitamin B9 (Biotin) in Food samples used in the application for Nutrition Testing

SKU: KBFP1527 Category:

The QUALICHEK Vitamin B9 (Biotin) ELISA includes features like:
– Ready to use protocol with break-apart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision

Validated against seven points for a “GOLD RING” Standard Quality ELISA – the benchmark sign for Krishgen Quality. The QUALICHEK ELISA kits are validated in the sample matrices indicsted. The kit uses recombinant antibodies to ensure a high degree of sensitivity and specificity in he assay.

Pack Type

1 x 96 wells, break-apart
Sample Matrix

Food
Interference

Preparations of the known factors were assayed for interference. No significant interference was observed.
Principle Of Assay

This ELISA is a sandwich immunoassay. Antibodies are coated on 96 well plates. The antigen protein present in sample and standard respectively bind to the coated wells. The wells are washed and an antibody:HRP Conjugate is added which binds to the bound complex in the well. Washing is performed to remove any unbound material. TMB substrate is added and the enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of antigen protein present in the standard or samples.
Detection Method

Colorimetric, Absorbance measured at 450nm
Conjugate-Enzyme Reaction

Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development. Our TMB formulation is chosen with appropriate kinetic properties to tailor our specific requirements, balancing speed, sensitivity, and dynamic range
Quality Certification

Validated as per KRISHGEN's Quality Program. We are and ISO9001, ISO13485 certified facility.
KRISHGEN Mandate

High Performance Assays at Affordable Prices
Regulatory Status

For Research Use Only (RUO).
Shipping Temperature

Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius
Storage Temperature

The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results, if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, well sealed and store at the indicated temperature. Similary aliquot and store the reconstituted standard at -20 Degree Celsius for long term storage (maximum 6 months from reconstitution) and to avoid deterioration in the activity.
Disclaimer

The data indicated herein with specifications are changed from time to time at time of production of the assay. We request you to confirm the specifications including the assay range and procedure as per the most current IFU (instructions for use) accompanying the assay kit.

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