QUALICHEK Aflatoxin B1 ELISA Kit (Milk and milk products)

QUALICHEK Aflatoxin B1 ELISA Kit (Milk and milk products)

₹31020

Enzyme Immunoassay for the estimation of Aflatoxin B1 ELISA Kit (TCM) in Grain/Feed samples used in the application for Grain Testing

SKU: KBFP1002-3 Category:

The QUALICHEK Aflatoxin B1 ELISA Kit (TCM) includes features like:
– Ready to use protocol with break-apart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision

Validated against seven points for a “GOLD RING” Standard Quality ELISA – the benchmark sign for Krishgen Quality. The QUALICHEK ELISA kits are validated in the sample matrices indicsted. The kit uses recombinant antibodies to ensure a high degree of sensitivity and specificity in he assay.

Pack Type

1 x 96 wells, break-apart
Sample Matrix

Grain/Feed
Sample Type

Milk, cheese, milk powder, milk beverage, and fermented milk.
Assay Range

0.05 – 1.5 ug/kg
Detection Limit

0; 0.046; 0.093; 0.187; 0.375; 0.75; 1.5 pg/mg
Sensitivity

1 ug/kg
Interference

Preparations of the known factors were assayed for interference. No significant interference was observed.
Principle Of Assay

This ELISA is a competitive inhibition assay (also called a competitive ELISA) is a powerful assay format used to detect small antigens or haptens that cannot be simultaneously bound by two antibodies (as in sandwich ELISA). In competitive ELISA, the sample / standard antigen competes with a labeled antigen for a limited amount of capture monoclonal antibody. The more antigen present in the sample, the less labeled antigen is bound, resulting in a lower signal. So, signal intensity is inversely proportional to the concentration of the target antigen.
Detection Method

Colorimetric, Absorbance measured at 450nm
Conjugate-Enzyme Reaction

Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development. Our TMB formulation is chosen with appropriate kinetic properties to tailor our specific requirements, balancing speed, sensitivity, and dynamic range
Quality Certification

Validated as per KRISHGEN's Quality Program. We are and ISO9001, ISO13485 certified facility.
KRISHGEN Mandate

High Performance Assays at Affordable Prices
Regulatory Status

For Research Use Only (RUO).
Shipping Temperature

Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius
Storage Temperature

The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results, if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, well sealed and store at the indicated temperature. Similary aliquot and store the reconstituted standard at -20 Degree Celsius for long term storage (maximum 6 months from reconstitution) and to avoid deterioration in the activity.
ELISA Type

Competitive ELISA
Application Area

analysis of the toxin residues in milk, cheese, milk powder, milk beverage, and fermented milk.
Disclaimer

The data indicated herein with specifications are changed from time to time at time of production of the assay. We request you to confirm the specifications including the assay range and procedure as per the most current IFU (instructions for use) accompanying the assay kit.

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