Enzymatic Assays:
All enzyme assays measure either the consumption of substrate or production of product over time. A large number of different methods of measuring the concentrations of substrates and products exist and many enzymes can be assayed in several different ways. We use colorimetric assays which follow the course of the reaction by measuring a change in how much light the assay solution absorbs. In this light is in the visible region and you can actually see a change in the color of the assay.
Rapid Immunochromatography:
Immunochromatography assay (ICA), namely lateral flow test, is a simple device intended to detect the presence or absence of the target analyte. The concept of immune-chromatography is a combination of chromatography (separation of components of a sample based on differences in their movement through a sorbent) and immunochemical reactions. The most widespread immunochromatographic system is the rapid test strip also known as rapid tests or rapid test devices.
ELISA:
The enzyme-linked immunosorbent assay is a commonly used analytical biochemistry assay. The assay uses a solid-phase type of enzyme immunoassay to detect the presence of a ligand in a liquid sample using antibodies directed against the protein to be measured. The end product is measured in a plate reader usually at a wavelength of 450nm or 630 nm.
